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Número do item:
145286292
Citrate Buffer 0.2 m, pH 4.0, 1 L
Número do item:
145286292
R$ 344
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Peça agora e receba por volta de Sexta, Julho 10
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Nossos principais parceiros logísticos
Citrate buffers can be used for RNA isolation and are a popular choice for immunofluorescent staining applications.
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Detalhes do produto
| Item Weight | 2.2 lbs (1 kg) |
DESCRIÇÃO DO PRODUTO
Citrate Buffer 0.2 m, pH 4.0, 1 L
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Perguntas e Respostas do cliente
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pergunta:
What is Citrate Buffer 0.2 m, pH 4.0 used for?
responda: Citrate Buffer 0.2 m, pH 4.0 is widely used in biological and biochemical applications, including enzyme reactions, protein purification, and maintaining the pH of samples in laboratory experiments. Its ability to stabilize pH levels makes it ideal for experiments requiring precise measurements, particularly in molecular biology techniques. For instance, in the process of electrophoresis, maintaining a stable pH is crucial to achieving accurate results. -
pergunta:
How is Citrate Buffer prepared?
responda: Citrate Buffer can be prepared by mixing citric acid and its sodium salts in a specific ratio to achieve the desired pH. For a 0.2 m concentration, carefully use a precise amount of citric acid and sodium citrate, followed by adjusting the pH with dilute sodium hydroxide or hydrochloric acid. Regular monitoring with a pH meter is essential to ensure accuracy. This preparation method is particularly useful in laboratory settings where customized buffers are essential for specific assays or reactions. -
pergunta:
Is Citrate Buffer suitable for cell culture applications?
responda: Yes, Citrate Buffer is suitable for various cell culture applications, especially when maintaining cell pH is crucial. Its phosphate-free formulation minimizes toxicity and supports the stable growth of sensitive cell lines. Researchers often use it during the preparation of culture media or during the maintenance phase to ensure optimal growth conditions, making it an essential component in cellular assays and biotechnology studies. -
pergunta:
What is the importance of pH 4.0 in Citrate Buffer?
responda: A pH of 4.0 in Citrate Buffer is significant because it falls within the optimal range for various biological reactions, particularly those involving enzymes that are active in acidic environments. This specific pH allows researchers to study enzyme kinetics, protein interactions, and other biochemical processes effectively. For example, many enzymes, such as pepsin, work best in acidic conditions, making this buffer ideal for experiments that require such environments. -
pergunta:
Can Citrate Buffer be used in protein analysis?
responda: Absolutely, Citrate Buffer is often employed in protein analysis, as it helps maintain a stable pH during the experimental procedure. Its compatibility with various proteins allows for effective analysis in techniques like chromatography and gel electrophoresis. Researchers frequently use this buffer to isolate and study proteins, as maintaining a consistent pH is crucial for preserving protein structure and function throughout the analysis. -
pergunta:
How should Citrate Buffer be stored?
responda: Citrate Buffer should be stored in a cool, dry place, ideally at room temperature and protected from direct sunlight. It is important to keep it in a tightly sealed container to prevent contamination and evaporation. Proper storage ensures its stability and effectiveness for later use, especially in critical laboratory experiments. Regular checks on the buffer’s clarity and pH are also advisable before each use to ensure optimal performance during experiments. -
pergunta:
What are the safety precautions when handling Citrate Buffer?
responda: When handling Citrate Buffer, it is essential to use personal protective equipment such as gloves and goggles to prevent skin or eye irritation. Although it's relatively safe, it's important to avoid ingestion and to work in a well-ventilated area. Always follow laboratory safety protocols, and check material safety data sheets (MSDS) for specific handling instructions. These precautions ensure safe use during various biochemical applications and experiments. -
pergunta:
Is Citrate Buffer compatible with other lab reagents?
responda: Citrate Buffer is generally compatible with a wide range of laboratory reagents, making it versatile for numerous applications. However, it's important to consider the specific chemistry of other reagents used in conjunction, as some compounds may alter the pH or stability of the buffer. Researchers often test compatibility beforehand, especially in multi-reagent experiments, ensuring that the buffer maintains its effectiveness while achieving desired outcomes. -
pergunta:
How does the concentration of Citrate Buffer affect its function?
responda: The concentration of Citrate Buffer directly influences its buffering capacity and effectiveness in maintaining pH during biochemical reactions. A 0.2 m concentration provides robust buffering ability, suitable for most biological applications. Adjusting the concentration allows researchers to tailor the buffer to specific experiments and conditions, ensuring that enzymatic reactions proceed optimally without significant pH fluctuations, which is critical for precise scientific work. -
pergunta:
Where can I buy Citrate Buffer 0.2 m, pH 4.0, 1 L in Brazil?
responda: You can purchase Citrate Buffer 0.2 m, pH 4.0, 1 L from Ubuy, which offers a reliable platform for sourcing this product in Brazil. Ubuy provides a user-friendly shopping experience with a variety of laboratory supplies at competitive prices.
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R$ 344
Peça agora e receba por volta de Sexta, Julho 10
Este item não é restrito no meu país. (Por favor clique no link acima se este item não for restrito em seu país para análise por parte da nossa equipe e permissão de envio).
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Transação Segura
A Ubuy trabalha para proteger sua segurança e privacidade. Nosso sistema avançado de segurança de pagamentos garante a confidencialidade ao criptografar suas informações durante a transmissão usando os protocolos AES (Advanced Encryption Standards) e SSL (Secure Socket Layer). Seus dados de pagamento estão 100% seguros, pois não compartilhamos suas informações com vendedores terceiros.
Nossos principais parceiros logísticos
A entrega internacional mais rápida
Características e benefícios
- High-quality Citrate Buffer, manufactured in an ISO certified facility.
- Ideal for RNA isolation by preventing base hydrolysis.
- Effective in antigen detection by breaking cross-links.
- Enhances stain intensity for immunofluorescent applications.
- Meets stringent Good Manufacturing Practices and quality control.
- Perfect for biological research and clinical applications.